This application note describes a method for analyzing three of the most popular artificial sweeteners in foods and beverages using the Agilent 1120 Compact LC system. The system suitability results showed that the Agilent 1120 Compact LC is the system of choice for conventional, analytical scale liquid chromatography. This integrated HPLC system was designed for ease of use, performance, and reliability. The quantitative analysis of typical samples is demonstrated in this Application Note.
This document describes an analysis method for three popular artificial sweeteners: acesulfame, saccharin, and aspartame. The method was developed to analyze these compounds in food and beverage samples, specifically yogurt and diet cola, to ensure compliance with regulations regarding their concentration.
The analysis was performed using the Agilent 1120 Compact LC system, an integrated HPLC system designed for ease of use, performance, and reliability. The system includes a gradient pump, autosampler, column compartment, and variable wavelength detector (VWD). The study demonstrates the system's capability to achieve precise retention times, peak areas, and low detection limits for routine quality control in the food industry.
| Mobile phase B | Acetonitrile |
|---|---|
| Flow rate | 1 mL/min |
| Wavelength | 214 nm |
| Temperature | 30 °C |
| Runtime | 11 minutes |
The analysis focused on three popular artificial sweeteners: acesulfame, saccharin, and aspartame.
The analysis was performed on samples of yogurt and a diet cola beverage.
The linearity was tested within the amount range from 18.75 to 1500 ng.