The Agilent 1120 Compact LC is the system of choice for conventional, analytical-scale liquid chromatography. It is an integrated LC designed for ease of use, performance, and reliability. It is well-suited for in-process control during drug manufacturing, due to the highly precise retention times and peak areas. This Application Note shows: Excellent retention time precision, with a relative standard deviation (RSD) < 0.07 %. Excellent area precision, with RSD < 0.25 % for baseline-separated peaks.
The document describes an HPLC method developed to analyze Aripiprazole and its precursors using reversed-phase high-performance liquid chromatography (RP-HPLC). The study highlights the system's suitability for monitoring levels of starting materials and by-products in drug manufacturing.
Experiments were conducted using the Agilent 1120 Compact LC system equipped with a gradient pump, autosampler, column compartment, and variable wavelength detector. Specific chromatographic conditions, including mobile phase composition, flow rate, and gradient profile, were established to achieve baseline separation of Aripiprazole from its starting materials.
The total run time, which includes re-equilibration, can be limited to 14 minutes.
The VWD was set to detect at a wavelength of 254 nm.
The system includes a column compartment capable of holding columns up to 250 mm in length.