This article introduces a method for separating 19 amino acids using an Agilent 1200 RRLC system, including two sulfurated amino acids, and transferring it successfully to an Agilent 1100 HPLC system. Good separation of this method was achieved by using Agilent ZORBAX Eclipse Plus C18 column on both two system. The results demonstrated that this analytical method was simple, reliable for analyzing these 19 amino acids, and the method is useful in comprehensively evaluating the quality of feed, food and pharmaceutical products which containing sulfurated amino acids.
The document details a reliable analytical method developed to separate 19 amino acids, specifically addressing the challenge of analyzing sulfurated amino acids alongside standard ones. The method utilizes pre-column derivatization with OPA and FMOC reagents.
The procedure was initially developed using an Agilent 1200 SL RRLC system and successfully transferred to an Agilent 1100 HPLC quaternary gradient system. Both systems utilize ZORBAX Eclipse Plus C18 columns, demonstrating the method's versatility across different LC configurations.
| Column (1200 SL RRLC) | 46x50 mm, 1.8um Zorbax Eclipse Plus C18 |
|---|---|
| Column (1100 HPLC) | 46x150 mm, 5 um Zorbax Eclipse Plus C18 |
| Flow Rate (1200 SL RRLC) | 2 mL/min |
| Flow Rate (1100 HPLC) | 2 mL/min |
| Column Temp. | 40°C |
The method analyzes a total of 19 amino acids, which includes two sulfurated amino acids (Methionine sulfone and Cysteic Acid) along with seventeen others.
The method uses OPA (Ortho-phthaldialdehyde) and FMOC (Fluorescein methyl ester carbamate) reagents.