Describes a method for analyzing star-branched polyethylene glycol. This method can be used with aqueous SEC.
This document illustrates the analysis of a star-branched polyethylene glycol containing a significant hydrophobic component using aqueous size exclusion chromatography (SEC). The study addresses the challenge of non-SEC effects caused by interactions between the hydrophobic sample and the column packing material.
To resolve these interactions, the sample was analyzed in a buffer at pH 7 both with and without the inclusion of 30% methanol. The results show that adding methanol significantly lowers the polarity of the eluent, minimizing hydrophobic interactions and yielding a normal Gaussian peak shape suitable for integration. The note confirms that Agilent PL aquagel-OH columns can effectively analyze such polymers when used with eluents containing up to 50% methanol.
| Column Part Number | PL1120-6830 |
|---|---|
| Column Dimensions | 300 x 7.5 mm |
| Flow Rate | 1.0 mL/min |
| Injection Volume | 100 uL |
| Maximum Methanol Content | 50% |
The presence of a miscible organic solvent such as methanol significantly lowers the polarity of the eluent and serves to minimize hydrophobic interactions.
Yes, these high performance columns can be used with up to 50 % methanol in the eluent.