This application note introduces a SEC method for the analysis of food-grade gelatins. Using Agilent PL aquagel-OH MIXED-H 8 µm columns, a wide molecular weight range can be analyzed with excellent resolution. This method simplifies the analysis of molecular weight distributions of water soluble polymers with intermediate to high molecular weight.
This document details a method for analyzing food-grade gelatins using size exclusion chromatography (SEC). The analysis utilizes Agilent PL aquagel-OH MIXED-H 8 um columns, which offer high performance and excellent resolution for water-soluble polymers with intermediate to high molecular weights. The setup includes an online light scattering detector calibrated with a pullulan polysaccharide standard to determine molecular weight distribution.
| Column Resolution Limit | up to 10,000,000 g.mol |
|---|---|
| Column Dimensions | 300 x 7.5 mm |
| Flow Rate | 1.0 mL/min |
| Sample Concentration | 1.0 mg/mL |
| Calibrant Molecular Weight | Mp 186,000 |
| Bulk Mw Value (90°) | 174,000 |
| Bulk Mw Value (15°) | 189,850 |
| Bulk Mw Value (SEC) | 184,800 |
Linear Agilent PL aquagel-OH MIXED-H 8 um columns are used.
It is calibrated using a pullulan polysaccharide standard with a known molecular weight of 186,000.
The eluent is water containing 0.2 M NaNO3 and 0.01 M NaH2PO4 at pH 7.