Agilent SEC Analysis of Dextrans Application Note

This application note introduces the use of Agilent PL aquagel-OH 40 and 60 8 µm columns for the analysis of dextrans.


Agilent SEC Analysis of Dextrans Application Note - cover page
Brand
Agilent
Category
Other
Document type
Application Note
Language
English
Pages
2
File format
PDF
File size
537 KB
Published
12 June, 2012
Updated
30 September, 2023
MD5 checksum
34C631539680A8F9E7A4A2DC37C739B6

About this manual

Dextran polysaccharides are used in medicine and various industries. This document details the use of Agilent PL aquagel-OH 40 and 60 8 um columns for analyzing dextran molecular size profiles via size exclusion chromatography (SEC). The columns were connected in series to cover a specific molecular weight range.

What's inside

  • Introduction
  • Conditions
  • Results and Discussion
  • Peak Identification
  • Conclusion

Specifications

Column TypePL aquagel-OH 40 and 60 8 um
Column Efficiency>35,000 plates/meter
Eluent0.2 M NaH2PO4 + 0.2 M NaCl at pH 7
Flow Rate1.0 mL/min
Detection MethodRI

Frequently asked questions

Which columns are ideal for SEC analysis of dextrans?

Agilent PL aquagel-OH 40 and 60 8 um columns are ideal for this purpose.

What is the molecular weight range covered by the connected columns?

The connected columns cover a molecular weight range from 10^4 to 10^6.

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