This application note introduces the use of Agilent PL aquagel-OH 40 and 60 8 µm columns for the analysis of dextrans.
Dextran polysaccharides are used in medicine and various industries. This document details the use of Agilent PL aquagel-OH 40 and 60 8 um columns for analyzing dextran molecular size profiles via size exclusion chromatography (SEC). The columns were connected in series to cover a specific molecular weight range.
| Column Type | PL aquagel-OH 40 and 60 8 um |
|---|---|
| Column Efficiency | >35,000 plates/meter |
| Eluent | 0.2 M NaH2PO4 + 0.2 M NaCl at pH 7 |
| Flow Rate | 1.0 mL/min |
| Detection Method | RI |
Agilent PL aquagel-OH 40 and 60 8 um columns are ideal for this purpose.
The connected columns cover a molecular weight range from 10^4 to 10^6.