This application note describes the best method for analyzing the molecular weight distribution of non-ionic surfactants using Agilent PL aquagel-OH 30 8 µm columns. The method provides high resolution and high reproducibility.
Non-ionic surfactants are used in various applications, and determining their molecular weight distribution is critical for performance. The document explains that Agilent PL aquagel-OH columns are ideal for these analyses due to their low exclusion limit, high pore volume, and high efficiency.
The note details the specific conditions required for analysis, including the addition of 30% methanol to the eluent to inhibit hydrophobic interactions. It provides specifications for column dimensions, flow rate, detection method (RI), and sample types.
| Column Efficiency | >35,000 plates/meter |
|---|---|
| Molecular Weight Range | 100 to 1,200,000 g/mol |
| Column Dimensions | 300 x 7.5 mm |
| Flow Rate | 1.0 mL/min |
| Detection Method | RI |
30% of a weak organic solvent (methanol) is required to inhibit hydrophobic interactions between the sample and the column packing.
Column calibration was achieved using polyethylene oxide Agilent EasiVial standards.