Agilent SEC Analysis of Sodium Polystyrene Sulfonate Application Note

About this manual

Sodium polystyrene sulfonate is an ion-exchange resin used to treat high levels of blood potassium. The document details a method for analyzing this sample using aqueous SEC with specific Agilent PL aquagel-OH columns designed for high pore volume and efficiency.

The analysis conditions include the use of 80% 0.3M NaNO3 + 0.01M Na2HPO4 at pH 9 mixed with 20% methanol as the eluent, flowing at 1.0 mL/min. Detection is performed using a Refractive Index (RI) detector.

What's inside

• Introduction
• Conditions
• Conclusion

Frequently asked questions

What is the stated use of sodium polystyrene sulfonate?

It is an ion-exchange resin used to treat high levels of blood potassium by substituting potassium in the diet and body for sodium.

What are the dimensions of the PL aquagel-OH columns?

The columns used have dimensions of 300 x 7.5 mm.

What is the flow rate for the analysis conditions?

The specified flow rate for the eluent is 1.0 mL/min.