This application note describes a water-based SEC method for the qualitative and quantitative analysis of food flavorings. The method uses Agilent PL aquagel-OH 30 8µm columns with RI detection in a pH 7 buffer. The method is suitable for the analysis of analytes with neutral, ionic, and hydrophobic moieties, either singly or in combination.
The document explains that modern foodstuffs often contain complex mixtures of chemicals, including low molecular weight maltodextrins and high molecular weight starch. Analyzing the relative amounts of these components is vital for quality control to ensure product consistency.
To perform this analysis, aqueous SEC was used with Agilent PL aquagel-OH 30 8um columns operating at pH 7 with RI detection. This setup allows investigators to focus on low molecular weight maltodextrins while excluding the less important high molecular weight starch from the analysis.
The flow rate is set to 1.0 mL/min.
The analysis is carried out in a buffer at pH 7.