This document introduces the characteristics of pectin as a gelling, thickening, and stabilizing agent used in the food industry. Pectin is a natural product extracted from plant raw materials such as apple, citrus, and beet. Pectins with specific properties can be obtained by processing the extracts. Although the chemical composition of pectin is key to its application, rheological behavior is critical to performance, and determination of the molecular weight distribution can help predict rheological behavior. SEC and Agilent PL aquagel-OH MIXED-H 8 µm columns are ideal for resolving pectins with their wide molecular weight resolving range (up to 10 million g/mol relative to PEO/PEG) and high efficiency (>35,000 plates/meter).
Pectin is a natural product used as a jellifying, thickening, and stabilizing agent in the food industry. Determining its molecular weight distribution is critical for predicting rheological behavior. Agilent PL aquagel-OH MIXED-H 8 um columns are ideal for this application due to their wide molecular weight resolving range and high efficiency.
The document details the experimental conditions used for the analysis, including sample preparation, column specifications, eluent composition, and flow rate. It presents chromatogram results and molecular weight averages for four different pectin samples, demonstrating the suitability of the columns for water-soluble polymers.
| Column Part Number | PL1149-6800 |
|---|---|
| Column Dimensions | 300 x 7.5 mm |
| Particle Size | 8 um |
| Flow Rate | 1.0 mL/min |
| Detection Method | Refractive Index (Ri) |
| Sample Concentration | 2 mg/mL |
Agilent PL aquagel-OH MIXED-H 8 um columns are ideal for resolving pectins.
The columns have a wide molecular weight resolving range up to 10 million g/mol relative to PEO/PEG.
Samples should be prepared at 2 mg/mL, left to fully dissolve overnight, and filtered through a 0.45 um membrane.