This application note describes a fast analytical method for the determination of residual polyacrylamides in reclaimed water using aqueous size exclusion chromatography (SEC). The method uses an Agilent PL aquagel-OH column to partially exclude the polymer peak, which increases the response and provides separation between the polymer and any lower molecular weight species that may be present in the sample.
This document details an analytical method for determining the concentration of residual polyacrylamides in reclaimed water. Polyacrylamides are commonly used as flocculants in water clarification, and monitoring their residual levels is critical for subsequent processing and environmental compliance. The note proposes using aqueous size exclusion chromatography (SEC) as a fast and effective technique for this purpose.
| Column | PL aquagel-OH 50 8 ym, 300 x 7.5mm |
|---|---|
| Sample Loading | 0.1 mg/mL, 200 pL |
| Eluent | 0.05 M Na2SO4 at pH 3 |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 208 nm |
Aqueous size exclusion chromatography (SEC) is used to quantify the amount of residual polymer.
It is added to stabilize the polymer solution and inhibit interaction between the polymer and the column packing material.