This document introduces the SEC method for measuring the molecular weight of hydroxyethyl cellulose using Agilent PL aquagel-OH 40 and 60 8 µm columns.
Hydroxyethyl cellulose (HEC) is a non-ionic polymer used in various industries. This document details how modifying its hydrophobicity alters molecular weight, which can be assessed using specific chromatography methods.
The analysis utilizes Agilent PL aquagel-OH 40 and 60 columns connected in series to cover a wide molecular weight range. Calibration is performed using pullulan standards with an RI detector.
| Column Efficiency | >35,000 plates/meter |
|---|---|
| Flow Rate | 1.0 mL/min |
| Detection Method | RI |
The analysis uses two PL aquagel-OH 60 8 ym columns and one PL aquagel-OH 40 8 ym column connected in series.
The flow rate used for the analysis is 1.0 mL/min.
Modifying the hydrophobicity alters the molecular weight of the hydroxyethyl cellulose.