This is an application note that describes the method to analyze polyacrylic acid by aqueous SEC using Agilent PL aquagel-OH columns.
This document details the analysis of polyacrylic acid, a resin formed by the polymerization of acrylic acid, using aqueous Size Exclusion Chromatography (SEC). The study utilizes Agilent PL aquagel-OH columns, which combine high pore volume and high column efficiency to provide maximum resolution for these polyelectrolytes.
The analysis was conducted using a salt/buffer eluent (0.25 M NaNO3 + 0.01 M NaH2PO4) at a flow rate of 1.0 mL/min with RI detection. The results demonstrate that SEC using these columns successfully provides molecular weight data, polydispersity information, and the shape of the molecular weight distribution.
| Column Pore Volume | high |
|---|---|
| Column Efficiency | >35,000 plates/meter |
| Column Dimensions | 300 x 7.5 mm |
| Particle Size | 8 pm |
| Flow Rate | 1.0 mL/min |
| Eluent Composition | 0.25 M NaNO3 + 0.01 M NaH2PO4 |
| Detection Method | RI |
The analysis used Agilent PL aquagel-OH 50 8 pm columns.
The eluent used was 0.25 M NaNO3 + 0.01 M NaH2PO4.
It provides molecular weight data, polydispersity, and the shape of the molecular weight distribution.