Agilent CP-Sil 88 for FAME GC Column Application Note

This document describes the separation of fatty acid methyl esters (FAME) on an Agilent J&W Select CP-Sil 88 column. The column has a high polarity liquid phase, which effectively differentiates between multiple FAME isomers.

Brand: Agilent
Category: Other Electronics
Document type: Application Note
Language: English
Pages: 3
File format: PDF
File size: 133 KB
Published: 12 June, 2012
Updated: 28 September, 2023
MD5 checksum: 2B0686C1B787F6D9DD88E5E68F67A988
PDF: Agilent CP-Sil 88 for FAME GC Column Application Note PDF

About this manual

This document describes the routine separation of fatty acid methyl esters (FAME) using the Agilent J&W Select CP-Sil 88 column. The column utilizes a stabilized, highly substituted cyanopropyl siloxane phase to effectively separate positional FAME isomers based on small structural differences. The application demonstrates that all 37 components of a complex FAME mixture can be resolved in a single run using GC-FID.

What's inside

Introduction
Conditions
Peak Identification
Column Specifications
Oven Program
Injection Parameters
Detection Parameters

Specifications

Column Length	100 m
Column Inner Diameter	0.25 mm
Film Thickness	0.2 um
Column Part Number	CP7489
Carrier Gas	Helium
Injection Temperature	250 °C
Injection Volume	0.5 yL
Detection Temperature	270 °C

Frequently asked questions

How many FAME components were resolved in the test?

All 37 components were resolved in one run.

What type of detector was used for the separation?

A Flame Ionization Detector (FID) was used.

What is the part number for the CP-Sil 88 for FAME column?

The part number is CP7489.

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