This note details the GC analysis of a 37-component FAME mixture using the Agilent J&W CP-Select for FAME column. The column is highly selective and durable, making it suitable for FAME identification purposes.
The document describes the GC analysis of fatty acids as their methyl esters derivatives (FAMEs). It highlights that the CP-Select for FAME column utilizes a fully crosslinked, high polarity liquid phase technology to resolve difficult cis/trans FAME isomers and offers improved durability over non-bonded columns. The note specifically details the separation results on this column using a standard 37-component FAME mixture.
| Column Length | 100 m |
|---|---|
| Column Internal Diameter | 0.25 mm |
| Part Number | CP7420 |
| Sample Volume | 1 yL |
| Carrier Gas Flow | 1 mL/min helium, constant flow |
| Injector Temperature | 220 °C |
| Detector Type | FID |
The part number for the CP-Select for FAME column is CP7420.
The analysis uses a sample volume of 1 yL with a concentration of approximately 250 ng/ul.
The temperature program ends with a hold at 250 °C for 15 minutes.