This document is an application note on Varian's 500-MS LC ion trap mass spectrometer. It discusses the use of Varian's Mass ID software for the deconvolution of biomolecules.
This document evaluates the Mass ID software for the deconvolution of multiply charged ions from proteins, peptides, and oligonucleotides analyzed on the Agilent 500-MS LC Ion Trap Mass Spectrometer. The study demonstrates the software's ability to determine accurate molecular weights with high mass accuracy for various biomolecules.
The evaluation covers the deconvolution of individual proteins, complex protein mixtures, and oligonucleotides in both desalted and un-desalted forms. The document also details the effectiveness of the software's background correction algorithm in handling low signal-to-noise spectra and its capability to resolve species with adducts.
| Column | Pursuit C18 column, 5 um, 150 mm x 2.1 mm ID |
|---|---|
| Injection volume | 10-100 ul |
| Flow rate | 0.2 ml/min |
| API Drying Gas | 15-30 psi at 150-350 °C |
| API Nebulizing Gas | 50 psi |
| Needle voltage | 3500-4500 V |
| Capillary voltage | 60-300 V |
| Shield voltage | 600 V |
| RF Loading | 80-100% |
The mass error for all tested proteins was found to be less than 0.014%.
Yes, the software can deconvolute species with adducts to their adducted accurate main mass.