This document describes the usage method and experimental conditions of the Beta Blockers Application 2002 LC Column, as well as the compounds detected and their peak identification. The LC column used is Polaris 3µ C18-A with dimensions of 50 x 2.0mm. The eluent consists of A: H2O + 0.1% NH4OH, pH 10.0 (concentration of 25 mM) and B: MeCN, with a gradient ratio of A:B 65:35. The flow rate is 0.6 ml/min, detection is done at UV @ 215nm, and the temperature is set at 40°C.
This document is an application note from Agilent Technologies regarding the analysis of beta blockers. It references Varian, Inc., which is now part of Agilent.
The note specifies the use of a Polaris 3u.C18-A column (Part Nr.: A2001050X020) with dimensions 50 x 2.0mm. The method involves an eluent mixture of water with 0.1% ammonium hydroxide at pH 10.0 and acetonitrile, with a flow rate of 0.6 ml/min and UV detection at 215nm.
| Column Part Number | A2001050X020 |
|---|---|
| Column Dimensions | 50 x 2.0mm |
| Flow Rate | 0.6 ml/min |
| Detection Wavelength | 215nm |
| Temperature | 40°C |
The application identifies pindolol, metoprolol, and propranolol.
The column part number is A2001050X020.