This application note describes the analysis of furfurals in wood sugar solutions obtained by low environmental impact hydrothermal treatment and mechanochemical treatment followed by enzyme-based glycosylation using the Agilent 1260 Infinity LC system.
This document details the analysis of furfural and 5-hydroxymethyl furfural (5-HMF) in wood sugar solutions obtained from non-edible plants like wood. The study focuses on samples treated via hydrothermal and mechanochemical methods followed by enzyme-based glycosylation.
The analysis was performed using the Agilent 1260 Infinity LC System, configured with a ZORBAX RRHT SB-C18 column and a Diode Array Detector. The document provides specific analytical conditions, including mobile phase composition, flow rate, and detection wavelengths, along with chromatograms and assay results for various biomass samples.
| Column | Agilent ZORBAX RRHT SB-C18 3.0 mm x 50 mm, 1.8 pm |
|---|---|
| Mobile phase | 0.1% formic acid/acetonitrile |
| Flow rate | 0.5 mL/min |
| Column temperature | 40 °C |
| Detection wavelength | 280 nm |
The analysis is performed using the Agilent 1260 Infinity LC System.
Furfural and 5-hydroxymethyl furfural (5-HMF) are produced as over-decomposition products.