Due to the current global acetonitrile shortage, Agilent Technologies has been investigating a range of alternative solvents that could be used for reversed phase HPLC analysis of peptides, which maintain the efficiency and selectivity that a PLRP-S 100Å 10 µm gives for this application when used with acetonitrile. This report details how a 385-LC ELS detector can be used to prevent the increased background absorbance of the mobile phase eluents when run on a UV detector. The 385-LC ELSD is capable of evaporating a wide range of different solvents, resulting in completely flat baselines and sharp peaks, even during gradient elution.
Due to global shortages, this document investigates alternative solvents for reversed phase HPLC peptide analysis. It details how switching from acetonitrile to methanol or ethanol can be achieved while maintaining separation efficiency using the Agilent PLRP-S 100Å column.
The primary focus is on utilizing the 385-LC ELS detector to overcome the high background absorbance and baseline drift caused by these alternative solvents in UV detection. The text provides specific gradient conditions, mobile phase compositions, and comparative data showing that the ELS detector produces flat baselines and sharp peaks comparable to acetonitrile-based runs.
The document tests methanol and ethanol as alternatives to acetonitrile.
The 385-LC ELSD is capable of evaporating a wide range of different solvents, resulting in completely flat baselines and sharp peaks.
The analysis uses an Agilent PLRP-S 100Å 10 µm column.