This application note describes a method for characterizing xylooligosaccharides derived from xylan using aqueous SEC with HPLC. The method provides accurate separation and characterization of oligomers with similar molecular weights, with excellent sensitivity and stability.
The document presents an analytical method for characterizing xylooligosaccharides, which are naturally occurring saccharides found in fruit, vegetables, honey, and milk. It highlights the commercial importance of these compounds due to their beneficial health properties and stability.
The note recommends Aqueous Size Exclusion Chromatography (SEC) combined with Evaporative Light Scattering Detection (ELSD) over conventional methods. Specifically, it details a system using PL aquagel-OH columns and the Agilent 380-ELSD detector to achieve high resolution for low molecular weight separations of sugar oligomers.
The system is an ideal solution for the characterization of xylooligosaccharides and other water-soluble sugar oligomers.
The procedure specifies a flow rate of 1.0 mL/min and an injection volume of 20 uL.
Yes, it can remove 100 % water at a flow rate of 5 mL/min at 30 °C.