1,12-Dodecyldiphosphonic acid is a phosphorous acid, is polar and has surfactant properties. It consists of two polar heads joined by a non-polar, twelve carbon chain. Agilent Technologies, Inc. published a application note to demonstrate how the Agilent ELSD can be used to analyze 1,12-dodecyldiphosphonic acid.
This document details a method for analyzing 1,12-dodecyldiphosphonic acid using High-Performance Liquid Chromatography (HPLC) coupled with an Evaporative Light Scattering Detector (ELSD). The compound is chosen because it lacks a chromophore for UV detection and is non-volatile, making the Agilent ELSD an ideal detector. The method utilizes a PLRP-S 100A column to separate the polar molecule effectively.
| Column | PLRP-S 100A 5 um, 150 x 4.6 mm |
|---|---|
| Detector Nebulizer Temperature | 60 °C |
| Detector Evaporator Temperature | 70 °C |
| Detector Gas Flow | 1.0 SLM |
| Flow Rate | 0.8 mL/min |
| Temperature | 60 °C |
| Sample Concentration | 1 mg/mL |
PLRP-S 100A columns are recommended because their small pore sizes have extremely high surface areas available to the solutes.
The analysis uses a flow rate of 0.8 mL/min.
The method requires operating at a temperature of 60 °C.