This report discusses the findings of an investigation into alternative solvents for the analysis of peptides on a SepTech ST150 10-C18
Due to a global shortage of acetonitrile caused by reduced demand for its raw materials, this document evaluates four alternative organic solvents: methanol, ethanol, iso-propanol, and n-propanol. The study aims to maintain the efficiency and selectivity provided by the SepTech ST150 10-C18 column while using these substitutes.
| Column Type | SepTech ST150 10-C18 |
|---|---|
| Flow Rate | 1 mL/min |
| Detection Wavelength (Acetonitrile) | 220 nm |
| Detection Wavelength (Methanol) | 280 nm |
Methanol was found to be the best alternative, providing separation similar to acetonitrile with greater spacing between peptides.
Methanol has a high UV response at 220 nm, so the separation must be run at 280 nm to avoid background absorbance issues.
Yes, iso-propanol and n-propanol need to be run at 40°C to reduce viscosity and back pressure.