This application note describes the optimization of an ion suppression reversed phase HPLC method to detect L-ascorbic acid, and use of the method in the quantitative determination of the acid in fruit juice. PLRP-S columns were used in this investigation. PLRP-S is a rigid macroporous styrene/divinylbenzene HPLC phase with outstanding chemical and physical stability, and high surface area. This chemical stability permits the use of low pH eluents and ion suppression reversed phase HPLC.
This document details the optimization of an ion suppression reversed phase High Performance Liquid Chromatography (HPLC) method to detect and quantify L-ascorbic acid. The study utilizes PLRP-S columns, which are described as rigid macroporous styrene/divinylbenzene phases with high chemical stability allowing for low pH eluents.
The optimized conditions involve a mobile phase of 0.2 M sodium dihydrogen phosphate adjusted to pH 2.14 with HCl. The method successfully resolves L-ascorbic acid from D-erythorbic acid and the solvent front within nine minutes, enabling accurate quantification in fruit juices without derivatization.
This document describes how to optimize an ion suppression reversed phase HPLC method to detect L-ascorbic acid and use it for quantitative determination in fruit juice.
The investigation uses a PLRP-S column with dimensions of 250 x 4.6 mm.
The specified flow rate for the method is 0.5 mL/min.