This application note describes a fast reversed-phase HPLC method for the determination of aspirin, salicylic acid and phenacetin. The method uses a highly retentive polymeric reversed-phase column (PLRP-S 100Å) and gradient elution. The method provides excellent separation of the analytes, and can be completed in less than 10 minutes. The method is also highly reproducible and stable.
This document presents an isocratic High-Performance Liquid Chromatography (HPLC) method designed to resolve the analgesics aspirin, salicylic acid, and phenacetin in under 10 minutes. The method addresses difficulties in quantifying these compounds when using standard UV detectors due to differing extinction coefficients.
The recommended system utilizes a PLRP-S 100A polymeric reversed phase column coupled with an Agilent evaporative light scattering detector (ELSD). This combination provides a uniform response for all analytes and allows the use of aggressive eluents across a wide pH range.
The method resolves aspirin, salicylic acid, and phenacetin in under 10 minutes.
The PLRP-S 100A column is 150 x 4.6 mm.
The nebulizer temperature is set to 95 °C and the evaporator to 50 °C.