This document introduces the method of using Agilent PLgel columns for GPC to analyze mono-, di-, and tristearoylglycerol.
Stearoylglycerols are esters of glycerol and stearic acid that can be produced as stable intermediates during microbial metabolism. This document details a straightforward method for analyzing these compounds using gel permeation chromatography (GPC) with Agilent PLgel columns.
The analysis successfully separates mono-, di, and tristearoylglycerol from stearic acid in less than 16 minutes using an Agilent PLgel 5 µm 50A column. The method utilizes THF as the eluent at a flow rate of 0.5 mL/min with an RI detector.
| Column | Agilent PLgel 5 µm 50A, 300 x 7.5 mm |
|---|---|
| Part Number | PL1110-6515 |
| Eluent | THF |
| Flow rate | 0.5 mL/min |
| Detector | RI |
| System | Agilent PL-GPC 50 |
The separation process takes less than 16 minutes.
The flow rate is 0.5 mL/min.
The eluent used is THF.