This document introduces the application of Agilent PLgel column and GPC/SEC technology in oligomer separation, as well as the effect of particle size on separation effect
This document provides a technical overview of the relationship between column particle size and separation resolution, as predicted by the Van Deemter equation. It demonstrates this effect using Agilent PLgel columns with three different particle sizes (3 µm, 5 µm, and 10 µm) to separate polystyrene standards.
The Agilent PLgel 100A column has dimensions of 300 x 7.5 mm.
The flow rate used for the separation is 1.0 mL/min.
The eluent used for the separation is THF.