This application note describes a trial of the purity and stability of stored L-ascorbic acid powder samples from four different manufacturers, and assesses degradation compounds and/or precursors from the production process.
This document details a study on the purity and storage stability of L-ascorbic acid powders from four different manufacturers, produced between 1971 and 1987. The study utilized ion suppression reversed phase HPLC with PLRP-S columns to analyze degradation compounds and precursors arising from the production process or storage conditions.
The analysis revealed a gradual decline in L-ascorbic acid purity with age, even in dry powder formulations. While aged samples showed lower purity and color changes, no specific degradation products or precursors were detected in the samples, suggesting alternative degradation mechanisms exist under near anaerobic conditions of low water activity.
| Flow rate | 0.5 mL/min |
|---|---|
| Detector | UV, 268 and 220 nm |
The study used ion suppression reversed phase HPLC with PLRP-S columns.
The flow rate used for the analysis was 0.5 mL/min.