This document discusses methods for improving the extraction and purification of pharmaceutical compounds, as well as the use of FlowTrap columns to address issues caused by TFA acid.
This document addresses the issue of compound isolation bottlenecks in the pharmaceutical industry caused by the evaporation of large volumes of HPLC fractions containing non-volatile eluents like trifluoroacetic acid (TFA). The text explains that TFA can pose risks of hydrolysis during evaporation and introduces FlowTrap columns as a solution for capturing and releasing small pharmaceutical molecules.
| Detection wavelength | UV 280 nm |
|---|---|
| Trapping Column dimensions | 4.6 x 150 mm |
| Column part number | PL1560-3M07 |
| Loading Eluent volume | 12 mL |
| Water Flow Rate | 4 mL/min |
| Base wash concentration | 2M NH3 |
| Elution solvent | 100% CH3CN |
The base wash protocol yielded freebase samples with no detectable TFA in the NMR analysis, removing more than 99% of TFA.
In situ freebasing results in a cleaner and narrower back elution peak profile, allowing for tighter elution volumes in sample collection.