A method for simultaneous determination of 11 β2-agonist residues of clebuterol, salbutamol, ractopamine, terbutaline, salmeterol, propranolol, tulobuterol, cimaterol, mabuterol, mapenterol, and zilpaterol in pork has been developed and validated. The analytes are extracted by liquid-liquid extraction (LLE) and solid-phase extraction (SPE) and quantified by liquid chromatography coupled to electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) operating in positive ion multiple reac- tion monitoring (MRM) mode. The method provides a pg/g level of limit of detection (LOD) for all β2-agonists in pork. The dynamic calibration ranges for these compounds are obtained from 0.25 to 5 ng/g. The overall recoveries range from 82% to 105% with RSD values between 1.6% and 8.4%.
The document presents an analytical method for the simultaneous determination of 11 beta-2 agonists (including clenbuterol, salbutamol, ractopamine, terbutaline, salmeterol, propranolol, tulobuterol, cimaterol, mabuterol, mapenterol, and zilpaterol) in pork. The method utilizes liquid-liquid extraction followed by solid-phase extraction (SPE) using Agilent Bond Elut Plexa PCX cartridges for purification.
| Limit of Detection (LOD) | pg/g level for all B2-agonists in pork |
|---|---|
| Dynamic Calibration Range | 0.25 to 5 ng/g |
| Overall Recoveries | 82% to 105% |
| RSD Values | between 1.6% and 8.4% |
The method determines clenbuterol, salbutamol, ractopamine, terbutaline, salmeterol, propranolol, tulobuterol, cimaterol, mabuterol, mapenterol, and zilpaterol.
Agilent Bond Elut Plexa PCX Solid-Phase Extraction Cartridges (60 mg, 3 mL) are used.
Agilent Poroshell 120 EC-C18, narrow bore, 2.1 x 100 mm, 2.7 µm.
The system operates in positive ion multiple reaction monitoring (MRM) mode.