Agilent Bond Elut Plexa polymeric SPE reduces ion suppression for better sensitivity and low LOD and LOQ. Beta blockers are extracted with excellent correlation coefficients, recoveries, and % RSD.
Ion suppression is a common issue in bioanalysis by LC/MS that leads to poor recovery and increased maintenance costs. This document presents evidence that the hydroxylated surface of Agilent Bond Elut Plexa reduces interaction with endogenous materials compared to competitor products with amide residues.
The study compares the performance of Agilent Bond Elut Plexa against two competitors using human plasma spiked with beta blockers. Results show that Bond Elut Plexa delivers higher sensitivity, lower limits of detection (LOD < 0.05 ng/mL), and excellent recovery rates with good linearity.
The product achieves a limit of detection (LOD) between 0.01 and 0.05 ng/mL.
The product achieves a limit of quantitation (LOQ) between 0.05 and 0.5 ng/mL.
All compounds showed a correlation coefficient greater than 0.995.