Agilent Bond Elut Plexa PCX is a cation exchange solid phase extraction product with hydroxylated surface, which can reduce the interaction between endogenous material in biological samples and the solid phase extraction sorbent, thereby reducing ion suppression and improving sensitivity.
This document is an application note for the Agilent Bond Elut Plexa PCX Cation Exchange Solid Phase Extraction (SPE) product. It addresses the issue of ion-suppression in liquid chromatography-mass spectrometry (LC/MS) bioanalysis, which can lead to low recovery and inaccurate results.
The text details an experiment comparing the Agilent Bond Elut Plexa PCX against two competitor products using a drug compound mixture spiked into blank plasma. The study highlights that the hydroxylated surface of the Agilent sorbent minimizes interaction with endogenous material, thereby reducing ion-suppression and improving sensitivity.
It reduces ion-suppression by minimizing interaction between the sorbent and endogenous material in biological matrices, resulting in improved sensitivity.
It features a hydroxylated surface rather than an amide residue on the sorbent's surface, which causes less interaction with endogenous material.
The study achieved low LODs ranging from 0.01 to 0.05 ng/mL and LOQs ranging from 0.05 to 0.5 ng/mL.