This document describes an effective, sensitive triple quadrupole LC/MS method with a simple, fast and economic sample preparation technique for the quantitation of 12 multi-plant growth regulators (PGR) in grape samples. Plant hormones or phytohormones are chemicals that regulate plant growth. Manmade compounds, or PGRs are used to regulate the growth of cultivated plants, weeds, and in vitro growth plants and plant cells. Synthetic PGRs are also used in various techniques such as cutting, grafting, and micropropogation. PGRs activate cellular responses such as cell death. Some have the ability to adversely affect human cancer cells. Because the European Union has listed these compounds, they are required to be measured.
This document details an effective and sensitive method for the quantitation of 12 multi-plant growth regulators (PGRs) in grape samples. The method utilizes a simple, fast, and economic dispersive C18 sample cleanup technique coupled with LC/MS analysis.
| Column | Agilent ZORBAX Extend C18 2.1 mm x 100 mm, 1.8 pm |
|---|---|
| Flow rate | 0.2 mL/min |
| Column temp | 40°C |
| Mobile phase A | Water {0.5% formic acid} |
| Mobile phase B | Methanol |
| Detection mode | Jet Stream ESI-LC/MS/MS in MRM mode |
The total run time required to determine 10 PGRs in positive mode is 16 min.
Chlormequat was detected at a 0.05 ppb level with good peak response.
The method uses a simple dispersive C18 cleanup process followed by direct injection.