This article describes the method of detergent removal using Agilent Bond Elut OMIX SCX pipette tips. The Bond Elut OMIX SCX pipette tips have good adsorption capacity and flow characteristics, which can quickly and effectively remove detergents.
This document details a simple and effective method for removing interfering detergents and salts from protein digests prior to mass spectrometry analysis. It highlights that while reversed-phase resins are effective for salt removal, strong cation exchange (SCX) materials like the Bond Elut OMIX SCX are much more proficient at removing detergents.
The note provides a step-by-step protocol for conditioning, binding, and washing samples using various methanol concentrations. Results demonstrate that washing solutions containing 50% to 100% methanol effectively remove detergents such as CHAPS, TritonX-100, and Tween20, enabling successful protein identification by MALDI-TOF MS.
| Part Number | A5700410 or A5700410K |
|---|---|
| Sample Volume | Up to 10 uL |
| Elution Solution | 5% ammonium hydroxide in 30% methanol |
A 50% methanol washing solution more effectively removed these detergents, resulting in better Mascot scores and sequence coverage.
Best removal of Tween20 was achieved with a 100% methanol washing solution.
Yes, larger sample volumes can be purified using Bond Elut OMIX SCX 100 uL tips.