This document introduces the solutions provided by Agilent Technologies in the food and agriculture field, including products, applications, software and services
This document serves as a promotional guide titled 'Excellent choices for food & agriculture applications' from Agilent Technologies. It highlights solutions for testing raw materials to final products in the dietary supplement industry, specifically focusing on identity, purity, quality, strength, and composition.
The text includes two specific technical examples: an analysis of triterpene glycosides in Black Cohosh tablets and a complex triglyceride analysis in Starflower (Borage) oil. Both applications utilize HPLC coupled with Varian Evaporative Light Scattering Detection (ELSD), which is noted for its universality and independence from the optical properties of the compounds being analyzed.
| Column (SI-01236) | C18 5 um, 150 x 4.6 mm |
|---|---|
| Detection (SI-01236) | Varian ELSD (neb=30 °C, evap=50 °C, gas=1.4 SLM) |
| Flow Rate | 1.0 mL/min |
| Injection Volume | 20 wL |
| Column (SI-01233) | C18 5 um, 250 x 4.6 mm |
| Detection (SI-01233) | Varian ELSD (neb=25 °C, evap=50 °C, gas=1.4 SLM) |
It is a better alternative because these compounds possess weak or no UV chromophores, which limits sensitivity with UV detection.
The primary active constituents are the triterpene glycosides actein, 27 deoxyactein and cimifugoside.
Varian ELSD is superior because it has better sensitivity than refractive index and is compatible with gradient elution.