This document describes the application of Agilent RapidFire/MS systems for high-throughput in vitro ADME analysis. Permeability assays such as Caco-2 and PAMPA are used to mimic the absorption of potential drug candidates by human intestinal cells. The Agilent RapidFire High-throughput Mass Spectrometry System, coupled with QQQ or TOF MS, enables rapid sample processing and provides results comparable to traditional LC/MS methods in a fraction of the time, making it a cost-effective and highly productive method for assessing permeability in the drug discovery process.
This document details the application of Agilent RapidFire/MS systems for conducting high-throughput in vitro ADME permeability assays, specifically using Caco-2 and PAMPA methods. The RapidFire system, interfaced with either a Q00 or Time-of-Flight (TOF) mass spectrometer, was utilized to analyze drug candidates efficiently.
The study compared the RapidFire system against traditional LC/MS/MS methods. Results showed that the RapidFire system achieved sample processing speeds of less than 10 seconds per sample, representing a ten-fold increase in throughput. Despite the speed, the permeability values obtained correlated strongly with those from LC/MS/MS, validating the system's accuracy for drug discovery workflows.
Samples are processed in less than 10 seconds each.
The system provides a 10-fold increase in throughput compared to conventional methods.
The study performed assays on a set of 81 proprietary drug discovery compounds.