This Application Note demonstrates the use of the Agilent 2100 Bioanalyzer and a dedicated Plant RNA assay to assess the integrity of plant RNA samples from multiple plant sources and differing degradative states.
High quality RNA is critical for functional genomics techniques like RT-PCR. The Agilent 2100 Bioanalyzer and its RNA Integrity Number (RIN) algorithm provide a user-independent assessment of RNA quality from minimal sample amounts. This document specifically demonstrates the use of a dedicated Plant RNA assay to assess plant RNA samples from various sources and degradative states.
The note details the validation of the assay using commercially available plant total RNA samples and a degradation study on tobacco tissue. It shows that the assay provides excellent precision with RIN values above 6.4 for high-quality samples and tracks the decrease in RIN as degradation progresses over time.
| Software Version | B.02.07 |
|---|---|
| Kit Protocol | RNA 6000 Nano kit |
| Sample Amount | picogram to nanogram |
| RIN Range | above 6.4 |
The assay allows for the assessment of plant RNA samples from multiple sources and differing degradative states.
It uses the RNA Integrity Number (RIN) algorithm to provide a user-independent assessment of total plant RNA integrity.
The coefficients of variation (CV) of the RIN measurements were in the range of 1.54% to 5.46%.