The document introduces an economical instrument configuration using the Agilent 7820A GC and 7697A headspace sampler for measuring ethanol in blood. It has good repeatability and easy-to-use operation, making it ideal for routine QA/QC labs. According to national standards, drunk driving is defined as a concentration of ethanol in the blood of 80 mg/100 mL or more, while drinking and driving is defined as a concentration of ethanol in the blood of 20 mg/100 mL or more. To meet this quantitative requirement, the calibration curve for the method was generated in a wide range of 10 mg/100 mL to 160 mg/100 mL, allowing both limits to be identified.
The document presents an economical instrument configuration consisting of the Agilent 7820 GC and 7697A headspace sampler for measuring ethanol in blood. It is designed for routine use in forensic laboratories, offering good repeatability and simple operation through full EPC control from auto-sampler to detector.
The note details a specific method meeting Chinese national standards (GB19522-2004 and GA/T842-2009) where drunk driving is defined as 80 mg/100 mL or more. The procedure involves preparing calibration curves in the range of 10 to 160 mg/100 mL using t-butanol as an internal standard, with specific gas chromatography and headspace sampler conditions provided.
Drunk driving is defined as a blood ethanol concentration of 80 mg/100 mL or more.
The calibration curve covers a range up to 160 mg/100 mL.