This application note introduces the use of the Agilent Select Biodiesel for Methanol column for the quantitative analysis of methanol in biodiesel. Using the Select Biodiesel for Methanol column, mixtures of biodiesel that contain other low boiling components can be analyzed with good reliability.
The document details a method for determining methanol content in fatty acid methyl esters (FAMEs) intended for use as pure biodiesel or blending components. It addresses the European Standard EN-14110, which specifies a concentration range from 0.01% to 0.5% (m/m) methanol, with requirements for automotive fuels (EN-14214) being less than 0.2% (m/m).
The procedure utilizes the internal standard method with isopropanol and the Agilent Select Biodiesel for Methanol column to achieve reliable separation from minor low-boiling compounds. The note provides specific instrument conditions, including column dimensions, injector and oven temperatures, carrier gas pressure, and detector settings. It also outlines the preparation of calibration solutions and reference samples, presenting results from the analysis of seven different biodiesel samples.
| Column dimensions | 0.32 mm x 30 m, 3 pm |
|---|---|
| Column part number | CP9083 |
| Injector temperature | 275 °C |
| Oven temperature | 40 °C |
| Carrier gas pressure | 80 kPa (11.6 psi) |
| Detector temperature | 300 °C |
| Headspace temperature | 80 °C |
| Average response factor | 1.18 |
The method is applicable for a concentration range from 0.01 % (m/m) to 0.5 % (m/m) methanol.
Requirements stated in EN-14214:2003 are less than 0.2 % (m/m) methanol.
Isopropanol (5 µL) is added to each vial as the internal standard.