The document introduces an automated sample preparation method for LC/MS/MS analysis of compounds in biological matrices using protein precipitation. The author presents a new protein precipitation filtration plate, Captiva ND, introduced by Agilent, which utilizes non-drip technology for simple operation and improved sample preparation efficiency.
Protein removal is a critical step in sample preparation for LC/MS/MS analysis. The document introduces the Agilent Captiva ND plate, which utilizes non-drip technology to enable fully automatable protein precipitation in a 96-well format. This method significantly reduces time and cost compared to traditional centrifugation techniques.
The method produces results in just a few minutes, which is five times faster than centrifugation methods.
The total time required for sample preparation is 5 minutes when based on automation.
The experiment involved over 5,000 continuous injections while monitoring system parameters.