This Application Note discusses the concept of dried matrix spot homogeneity and demonstrates the homogeneity of blood spots on an Agilent Bond Elut DMS card. Results show that the size of the blood spots and the analyte dispersion is homogeneous, and the recoveries are reproducible regardless of whether the sample is taken from the center or the edge of the blood spot.
Dried blood spotting is an alternative to traditional plasma extraction for toxicokinetic and pharmacokinetic data. This document investigates whether the size of the blood spot and the dispersion of analytes are consistent across different locations within the spot.
Four pharmaceutical analytes (clozapine, zolpidem, paroxetine, and nortriptyline) were tested using 20 µL drops of spiked human blood on Agilent Bond Elut DMS cards. The results demonstrated that spot diameters had an RSD of 3% or lower across three different lots, and analyte recoveries were consistent regardless of whether the sample was punched from the center or the edge.
The RSD values for the diameter of the spots were all 3% or lower.
No, they are intended only for DMPK/ADME research applications.