The method developed in this work was based on immunoaffinity clean-up and electrochemical derivatization. The method showed good linearity (0.1-10 µg/L), sensitivity (LOD as low as 0.004-0.007 µg/L), and precision (RSDs less than 0.1% and 0.3%). It can be used for the simultaneous determination of four aflatoxins in corn and peanut butter.
The document details an analytical method developed by Agilent Technologies for the simultaneous determination of four aflatoxins (B1, B2, G1, and G2) in corn and peanut butter samples. The procedure utilizes High-Performance Liquid Chromatography coupled with Fluorescence Detection (HPLC-FLD).
The method maintains linearity over a concentration range of 0.1 to 10.0 pg/L for each aflatoxin.
The analysis time was set for 12 minutes to ensure complete elution of all components in the sample.