Agilent J&W DB-35ms Ultra Inert GC Column Application Note for TSNA Analysis

About this manual

The document presents a simple and sensitive method developed by Shanghai Tobacco Corp. and Agilent Technologies for the routine analysis of carcinogenic tobacco-specific nitrosamines (TSNAs) in mainstream cigarette smoke. The study focuses on compounds such as NNN, NNK, NAT, and NAB.

The methodology involves collecting smoke particulate matter according to ISO 4387, extracting it with methylene chloride, cleaning up the sample using an Agilent Bond Elut Alumina B SPE cartridge, and analyzing it via Gas Chromatography/Mass Spectrometry (GC/MS). The application highlights the use of the Agilent J&W DB-35ms Ultra Inert GC column to ensure accurate quantitation due to its low bleed and high inertness, which prevents peak tailing common with active TSNAs.

What's inside

• Abstract
• Introduction
• Experimental
• Instrumentation
• Results and Discussion
• Column selection
• Method performance

Frequently asked questions

What is the method detection limit for NNK in mainstream cigarette smoke using this method?

The estimated method detection limit for NNK is 0.03 ng/cig.

What is the lower limit of quantitation for NNK in this analysis?

The lower limit of quantitation for NNK is 0.087 ng/cig.

What is the range of recovery percentages achieved for NNK in blank matrix spikes?

The recovery results ranged from 98.8% to 100.2%.