This document describes a method for analyzing antibiotics in meat using an Agilent Poroshell 120 EC-C18 column. The method uses a gradient method and UV detection, and it can be quickly optimized.
The document details the transfer of an existing analytical method originally designed for UV detection to a mass spectrometry (MS/MS) compatible protocol. The primary goal is to analyze antibiotics such as sulfamerazine and thiamphenicol in meat matrices more efficiently.
The study utilizes Agilent Poroshell 120 EC-C18 columns, which allow for rapid method optimization using short gradient times compared to traditional long columns. The optimized method was successfully demonstrated on an Agilent 6410 triple quadrupole LC/MS System coupled with an Agilent 1200 Series Rapid Resolution LC.
| Column Dimensions (Short) | 4.6 mm x 50 mm, 2.7 um |
|---|---|
| Column Dimensions (Medium) | 3.0 mm x 50 mm, 2.7 um |
| Column Dimensions (Long) | 2.1 mm x 100 mm, 2.7 um |
| Gradient Time (Optimized) | 12 min |
| Flow Rate (Short Column) | 2 mL/min |
An Agilent 6410 triple quadrupole LC/MS System coupled with an Agilent 1200 Series Rapid Resolution LC.
MS-compatible mobile phases consisting of volatile buffer components such as formic acid, ammonium formate buffer and ammonium acetate buffer.