This application note describes LC-MS/MS methods for the determination of Alternaria toxins in vegetables and fruit beverages.
This document details an analytical method for the qualitative and quantitative determination of five important alternaria toxins (AOH, AME, ALT, TEN, TEA) in vegetable and fruit beverage matrices. The method utilizes electrospray ionization LC-tandem mass spectrometry with positive/negative ion detection in MRM mode to achieve high sensitivity at sub-ng/mL levels.
The procedure involves sample extraction using acetonitrile-methanol-water mixtures, followed by cleanup and concentration via solid phase extraction (SPE) using Agilent's Bond Elut Plexa hydroxylated polymer. Separation is performed on an Agilent MonoChrom MS silica gel column with a pentafluorophenyl modification.
| Column Type | Agilent MonoChrom MS silica gel column, 5 µm, 150 x 3 mm |
|---|---|
| Column Part Number | A2080150X030 |
| SPE Cartridge Type | Bond Elut Plexa 200 mg, 6 mL cartridge |
| SPE Cartridge Part Number | 12109206 |
| Limit of Detection (LOD) | >5 µg/L |
| Limit of Determination (LOQ) | >10 pg/L |
The method determines alternariol (AOH), alternariol monomethyl ether (AME), altenuene (ALT), tentoxin (TEN), and tenuazonic acid (TEA).
The method covers liquid samples such as grape juice and vegetable beverages, as well as solid samples like fruit purées.
The application recommends using a Bond Elut Plexa 200 mg, 6 mL cartridge.