Agilent J&W FactorFour VF-5ms Column Application Note for Fast GC/MS/MS of Androgenic Anabolic Steroids

A fast GC/MS/MS method for the screening of 13 anabolic androgenic steroids with high specificity and short analysis time.

Brand: Agilent
Document type: Application Note
Language: English
Pages: 5
File format: PDF
File size: 416 KB
Published: 11 June, 2012
Updated: 30 September, 2023
Model: VF-5MS
MD5 checksum: D3BE051665A5064C87C6177A7D5F1D5D
PDF: Agilent J&W FactorFour VF-5ms Column Application Note for Fast GC/MS/MS of Androgenic Anabolic Steroids PDF

About this manual

This document details a rapid screening method for detecting 13 anabolic androgenic steroids in urine samples. The method utilizes a short VF-5ms GC column combined with multiple reaction monitor (MRM) detection to achieve high specificity and speed, completing the analysis in approximately 12 minutes.

What's inside

Introduction
Sample Preparation
Conditions
Results
Conclusion

Specifications

Column	VF-5ms, 10 mx 0.15 mm x 0.15 um
Part Number	CP9034
Cartridge	BondElut C18, 200 mg
Carrier Gas	0.5 mL/min Helium
Injector Temperature	250 °C
Detector	Triple quadripole GC
Ion Source Temperature	250 °C
Analysis Time	12 minutes

Frequently asked questions

How many anabolic steroids can this method screen?

The method is designed to screen 13 anabolic androgenic steroids.

What is the total analysis time for the method?

The analysis is completed within 12 minutes.

What internal standard is used in the sample preparation?

17a-methyltestosterone is added as an internal standard.

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