This application note compares the UV sensitivity and detection limits of the Agilent 1260 Infinity Analytical SFC System and the Agilent 1200 Series LC System. Two applications were analyzed using the same column, detector, and separation conditions for both SFC and RPLC. The results showed that although the Agilent 1200 Series LC System was slightly more sensitive, the Agilent 1260 Infinity Analytical SFC System achieved near-HPLC sensitivity.
This document compares the UV sensitivity and detection limits of the Agilent 1260 Infinity Analytical SFC System against an Agilent 1200 Series LC System. The comparison utilizes two specific applications: a standard mixture of polycyclic aromatic hydrocarbons (PAHs) and the determination of 2,6-dichloroaniline, a potential genotoxic impurity, in diclofenac. To ensure a fair comparison, identical columns, injection volumes, detectors, and flow cells were used for both supercritical fluid chromatography (SFC) and reversed phase liquid chromatography (RPLC) modes.
| Outlet pressure (SFC) | 120 bar |
|---|---|
| Flow rate | 2.0 mL/min |
| Temperature (SFC) | 40 °C |
| Publication Number | 5990-9195EN |
The Agilent 1200 Series LC System was slightly more sensitive than the SFC system.
Typically, the limits of detection in LC mode are between two and four times lower than in SFC mode.
The limit of quantification was below the 0.01% impurity level (S/N > 13).