This application note demonstrates the value of the VF-624ms GC column with EZ-Guard in the analysis of Class 2 solvents according to the USP 467 method.
The document describes an analytical method for testing residual solvents in drug substances according to US Pharmacopeia General Chapter 467. It focuses on the analysis of Class 2 solvents, which are considered to have limited use due to toxicity concerns.
The study utilizes Agilent J&W FactorFour VF-624ms gas chromatography columns equipped with an integrated EZ-Guard column. The text details the specific materials and methods used, including sample concentrations, temperature programs, carrier gas settings, and detector temperatures. Results show symmetrical peak shapes for various solvents in Mix A and Mix B samples.
The document demonstrates the separation of USP 467 Class 2 solvents, which are grouped into Mix A and Mix B.
The recommended sample size is 0.2 pL.