This document investigates the enantiomer separation of six profen drugs using Daicel immobilized polysaccharide-derived chiral columns and the Agilent 1260 Infinity Analytical SFC System. Methanol, ethanol, 2-propanol, and acetonitrile were used in combination with trifluoroacetic acid as the mobile phase modifier in the supercritical CO2. Systematic studies were conducted to achieve complete resolution of these profen drugs on the given column set by adjusting the mobile phase additive, modifier nature, and its percentage.
This document investigates the analytical separation feasibility of six profen drugs (Flurbiprofen, Ibuprofen, Ketoprofen, etc.) using the Agilent 1260 Infinity Analytical SFC System. The study utilizes a set of columns packed with immobilized polysaccharide-derived chiral stationary phases (CSPs) from Daicel Corporation, specifically CHIRALPAK IA, IB, IC, and ID.
The note systematically evaluates the effects of mobile phase modifiers (methanol, ethanol, 2-propanol, acetonitrile), the addition of trifluoroacetic acid (TFA), and modifier percentages on separation resolution. It concludes that automated method development with the specified system is fast and highly successful for achieving complete enantiomer resolution of these compounds.
| Column dimensions | 4.6 x 150 mm |
|---|---|
| Flow rate | 3.0 mL/min |
| Column temperature | 35 °C |
| TFA concentration | 0.06% by volume |
The study used CHIRALPAK IA, CHIRALPAK IB, CHIRALPAK IC, and CHIRALPAK ID columns manufactured by Daicel Corporation.
The addition of 0.06% TFA in the mobile phase resulted in shorter retention times, higher enantioselectivity, increased plate counts, and better peak symmetry.
2-PrOH (2-propanol) provided the highest resolution for Carprofen enantiomers, achieving an Rs of 7.38.