Ion exchange is a commonly used technique for the separation of complex protein mixtures. This application note demonstrates how analysis times can be significantly reduced, increasing throughput without compromising analytical performance, by exploiting the benefits of small particle size, non-porous ion exchange sorbents.
This document is an application note from Agilent Technologies regarding the use of Weak Cation Exchange (WCX) columns for separating complex protein mixtures. It highlights that traditional methods often require long column lengths and shallow gradients, which can be time-consuming.
The text explains that using non-porous ion exchange sorbents with smaller particle sizes allows for significantly reduced analysis times without compromising analytical performance. The note details specific Agilent Bio WCX NP models (NP5, NP3, NP1.7) and compares their separation efficiency against traditional porous particles, showing how shorter columns packed with smaller particles can achieve high resolution in minutes rather than hours.
The document states that a flow rate of 1.7 mL/min keeps the backpressure below 400 bar.
The Bio WCX NP5 column has dimensions of 4.6 x 250 mm SS and a part number of 5190-2445.
The procedure specifies an injection volume of 10 pL.