The document describes the use of the Agilent 6550 iFunnel Q-TOF LC/MS system for high-throughput, high-efficiency metabolome profiling. Compared to the previous generation Q-TOF instrument, this platform has increased sensitivity and resolution, resulting in the detection of an order of magnitude more ions. It provides a 6 to 7-fold increase in coverage of the E. coli metabolome and more detailed pathway analysis.
This document describes a method for high-throughput, high-efficiency metabolome profiling using the Agilent 6550 iFunnel Q-TOF LC/MS System coupled with flow injection analysis (FIA). The study analyzes small molecules in biological extracts, specifically E. coli, to demonstrate the system's superior performance compared to previous generation instruments.
The application note details the experimental setup, including the use of an Agilent 1290 Series LC pump and Wellplate Autosampler. It presents results showing that the 6550 system detects an order of magnitude more ions than the 6520 platform, leading to a six to seven-fold increase in coverage of the E. coli metabolome and enabling MS/MS identification at concentrations as low as 0.006 pM.
| Resolving power | 40,000 |
|---|---|
| Mass accuracy | < 1 ppm |
| Detection limit | 0.006 pM |
| Flow rate | 150 uL/min |
| Drying gas temperature | 325 C |
| Capillary voltage | 3,500 V |
| Fragmentor voltage | 360 V |
| Skimmer voltage | 65 V |
| Oct RF Vpp | 750 V |
| Acquisition range | 50-1,000 m/z |
The system enables MS/MS identification of compounds at concentrations as low as 0.006 pM.
The 6550 system detects an order of magnitude more ions than the 6520 platform.
The 6550 system provides a six to seven fold increase in coverage of the E. coli metabolome.